Quick Overview for IgA ELISA Kit (ABIN454024)
Target
See all IgA ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- The IgA(pig)-ELISA is designed for the quantitative determination of pig IgA in complex samples (serum or other biological samples).
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Analytical Method
- Quantitative
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Characteristics
- The determination of pig IgA is carried out as direct sandwich ELISA.
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IgA ELISA Kit
Reactivity: Cow Colorimetric Competition ELISA 0.39 μg/mL - 25 μg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
IgA ELISA KitReactivity: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
IgA ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
IgA ELISA KitReactivity: Human Colorimetric Sandwich ELISA 0.14 ng/mL - 100 ng/mL Plasma, Saliva, Serum
IgA ELISA KitReactivity: Sheep Colorimetric Competition ELISA 3.75 μg/mL - 60 μg/mL Plasma, Serum
IgA ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
IgA ELISA KitReactivity: Chicken Colorimetric Competition ELISA 781.25 ng/mL - 50000 ng/mL Plasma, Serum
IgA ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
IgA ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
IgA ELISA KitReactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
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Plate
- Pre-coated
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Protocol
- An antibody specific for pig IgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgA present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgA concentration.
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Assay Procedure
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm -
Assay Precision
- Intraassay precision (CV):(n=10)at 25 ng/ml: 6.6%at 100 ng/ml: 4.3%at 237 ng/ml: 7.9%
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- IgA
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Target Type
- Antibody
Target See all IgA ELISA Kits
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