IgY ELISA Kit
Quick Overview for IgY ELISA Kit (ABIN454026)
Target
See all IgY productsReactivity
Detection Method
Method Type
Application
Sample Type
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Minimum Detection Limit
- 80 ng/mL
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Purpose
- The IgG(chicken)-ELISA is designed for the quantitative determination of chicken IgG in complex samples (serum or other biological samples).
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Analytical Method
- Quantitative
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Characteristics
- The determination of chicken IgG is carried out as direct sandwich ELISA.
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IgY ELISA Kit
Reactivity: Chicken Colorimetric Sandwich ELISA 0.078 ng/mL - 5 ng/mL Egg yolk, Plasma, Serum
IgY ELISA KitReactivity: Chicken Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
IgY ELISA KitReactivity: Chicken Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgY ELISA KitReactivity: Chicken Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- An antibody specific for chicken IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
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Assay Procedure
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm -
Assay Precision
- Intraassay precision (CV):(n=10)at 50.8 ng/ml: 3.0%at 122.6 ng/ml: 6.6%
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- IgY
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Alternative Name
- IgG/IgY
Target See all IgY products
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