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IgG ELISA Kit

Reactivity: Rabbit Colorimetric Sandwich ELISA Serum
Catalog No. ABIN454028
  • Target See all IgG ELISA Kits
    IgG
    Reactivity
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    Rabbit
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Minimum Detection Limit
    156 ng/mL
    Application
    ELISA
    Purpose
    The IgG(rabbit)-ELISA is designed for the quantitative determination of rabbit IgG in complex samples (serum or other biological samples).
    Sample Type
    Serum
    Analytical Method
    Quantitative
    Characteristics
    The determination of rabbit IgG is carried out as direct sandwich ELISA.
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  • Plate
    Pre-coated
    Protocol
    An antibody specific for rabbit IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
    Assay Procedure

    Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
    Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
    Wash Substrate incubation: 100 µL/well 10 min, room temperature
    Add stop solution: 100 µL/well
    Read at 450 nm

    Assay Precision
    Intraassay precision (CV):(n=10)at 4.1 ng/ml: 2.9%at 17.3 ng/ml: 2.5%at 34.7 ng/ml: 2.6%
    Restrictions
    For Research Use only
  • Storage
    4 °C
  • Target See all IgG ELISA Kits
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
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