Aquaporin 1 ELISA Kit

Catalog No. ABIN454453

Human Aquaporin 1 ELISA Kit, Colorimetric assay for quantification of Human Aquaporin 1.

Quick Overview for Aquaporin 1 ELISA Kit (ABIN454453)

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156-10 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma

Product Details Aquaporin 1 ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: This immunoassay kit allows for the specific measurement of human AQP1 concentrations in cell culture supernates, tissue homogenates serum, and plasma.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural human AQP1.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 0.04 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Homo sapiens,Human,Aquaporin-1,AQP-1,Aquaporin-CHIP,Urine water channel,Water channel protein for red blood cells and kidney proximal tubule,AQP1,CHIP28

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for AQP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AQP1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for AQP1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AQP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Alternative Name: AQP1

Background: AQP1 is a polypeptide hormone produced in pancreatic beta-cells that belongs to the family of calcitonin gene-related peptides. There is a 20% sequence homology between AQP1 and calcitonin and 44% homology with calcitonin gene-related peptide. AQP1 and its fragments stimulate the proliferation of osteoblasts, inhibit bone resorption, and increase bone density and the amount of bone mass.

Pathways: Hormone Transport