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Aquaporin 1 ELISA Kit

AQP1 Reactivity: Human Colorimetric Sandwich ELISA 0.156-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN454453
  • Target See all Aquaporin 1 (AQP1) ELISA Kits
    Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))
    Reactivity
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    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156-10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Purpose
    This immunoassay kit allows for the specific measurement of human AQP1 concentrations in cell culture supernates, tissue homogenates serum, and plasma.
    Sample Type
    Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma
    Analytical Method
    Quantitative
    Specificity
    This assay recognizes recombinant and natural human AQP1.
    Cross-Reactivity (Details)
    No significant cross-reactivity or interference was observed.
    Sensitivity
    < 0.04 ng/mL
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    Characteristics
    Homo sapiens,Human,Aquaporin-1,AQP-1,Aquaporin-CHIP,Urine water channel,Water channel protein for red blood cells and kidney proximal tubule,AQP1,CHIP28
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for AQP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AQP1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for AQP1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AQP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
    Storage Comment
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • Target See all Aquaporin 1 (AQP1) ELISA Kits
    Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))
    Alternative Name
    AQP1 (AQP1 Products)
    Synonyms
    fj33g04 ELISA Kit, zgc:85890 ELISA Kit, wu:fj33g04 ELISA Kit, AQP1 ELISA Kit, CHIP28 ELISA Kit, AQP-CHIP ELISA Kit, CO ELISA Kit, AQP-1 ELISA Kit, CHIP29 ELISA Kit, aquaporin 1a (Colton blood group), tandem duplicate 1 ELISA Kit, aquaporin 1 ELISA Kit, aquaporin 1 (Colton blood group) ELISA Kit, aqp1a.1 ELISA Kit, AQP1 ELISA Kit, Aqp1 ELISA Kit
    Background
    AQP1 is a polypeptide hormone produced in pancreatic beta-cells that belongs to the family of calcitonin gene-related peptides. There is a 20% sequence homology between AQP1 and calcitonin and 44% homology with calcitonin gene-related peptide. AQP1 and its fragments stimulate the proliferation of osteoblasts, inhibit bone resorption, and increase bone density and the amount of bone mass.
    Pathways
    Hormone Transport
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