LDHD ELISA Kit
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- Target See all LDHD ELISA Kits
- LDHD (Lactate Dehydrogenase D (LDHD))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of Mouse D-LDH, concentrations in serum, tissue homogenates and other biological fluids.
- Sample Type
- Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay recognizes recombinant and natural Mouse D-LDH.
- Cross-Reactivity (Details)
- No significant cross-reactivity or interference was observed.
- Sensitivity
- The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
- Characteristics
- Mus musculus,Mouse,Probable D-lactate dehydrogenase, mitochondrial,DLD,Lactate dehydrogenase D,Ldhd,1.1.2.4
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Lactate Dehydrogenase D (LDHD) ELISA Kit
LDHD Reactivity: Rat Colorimetric Sandwich ELISA 0.16 pmol/mL - 10 pmol/mL Plasma, Serum
Lactate Dehydrogenase D (LDHD) ELISA KitLDHD Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Lactate Dehydrogenase D (LDHD) ELISA KitLDHD Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Lactate Dehydrogenase D (LDHD) ELISA KitLDHD Reactivity: Mouse Colorimetric Sandwich ELISA 4.69 mU/mL - 300 mU/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-LDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for D-LDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of D-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
- Storage Comment
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Target See all LDHD ELISA Kits
- LDHD (Lactate Dehydrogenase D (LDHD))
- Alternative Name
- Ldhd (LDHD Products)
- Synonyms
- DLD ELISA Kit, 4733401P21Rik ELISA Kit, D8Bwg1320e ELISA Kit, Ac2-202 ELISA Kit, lactate dehydrogenase D ELISA Kit, LDHD ELISA Kit, ldhd ELISA Kit, Ldhd ELISA Kit
- Pathways
- Ribonucleoside Biosynthetic Process
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