CCL24 ELISA Kit

Catalog No. ABIN455041

Mouse CCL24 ELISA Kit, Colorimetric assay for quantification of Mouse CCL24.

Quick Overview for CCL24 ELISA Kit (ABIN455041)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Product Details CCL24 ELISA Kit

Purpose: This immunoassay kit allows for the specific measurement of mouse Eotaxin-2 (CCL24) concentrations in cell culture supernates, serum, plasma , BALF and tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse CCL24. The factors listed below were assayed for cross-reactivity.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Characteristics: Mus musculus,Mouse,C-C motif chemokine 24,Eosinophil chemotactic protein 2,Eotaxin-2,Small-inducible cytokine A24,Ccl24,Scya24

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CCL24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL24 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for CCL24 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL24 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for CCL24

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Alternative Name: Ccl24

Background: Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and Il-4. LPS and IL-4 also differentially regulate the expression of eotaxin-2 in monocytes and macrophages. Mouse eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human eotaxin-2. Functionally, eotaxin-2 is most closely related to eotaxin/CCL11 and eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin.

Pathways: Regulation of Actin Filament Polymerization