FSHB ELISA Kit

Catalog No. ABIN455139

The Human FSHB ELISA Kit (ABIN455139) is a Colorimetric ELISA Kit designed to quantify Human FSHB. This ELISA Kit has been cited in 1 publication.

Quick Overview for FSHB ELISA Kit (ABIN455139)

Target: FSHB (Follicle Stimulating Hormone, beta Polypeptide (FSHB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 MioU/mL

Application: ELISA

Sample Type: Serum, Plasma

Product Details FSHB ELISA Kit

Minimum Detection Limit: 1.56 MioU/mL

Purpose: This immunoassay kit allows for the specific measurement of Human Follicle-Stimulating Hormone (FSH) concentrations in serum and plasma.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural Human FSH.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Homo sapiens,Human,Follitropin subunit beta,Follicle-stimulating hormone beta subunit,FSH-B,FSH-beta,Follitropin beta chain,FSHB

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for FSH is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color 2 develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

References for FSHB ELISA Kit (ABIN455139)

Lv, Guo, Shi: "Effects of quinestrol on reproductive hormone expression, secretion, and receptor levels in female Mongolian gerbils (Meriones unguiculatus)." in: Theriogenology, Vol. 77, Issue 6, pp. 1223-31, (2012) (PubMed).

Target Details for FSHB

Target: FSHB (Follicle Stimulating Hormone, beta Polypeptide (FSHB))

Alternative Name: FSHB

Background: Follicle stimulating hormone (FSH) is a hormone synthesised and secreted by gonadotropes in the anterior pituitary gland. In the ovary FSH stimulates the growth of immature Graafian follicles to maturation. FSH is a glycoprotein secreted by the basophil cells of the anterior pituitary. Gonadotropin-releasing hormone (GnRH), produced in the hypothalamus, controls the release of FSH from the anterior pituitary. Like other glycoproteins, such as LH, TSH, and HCG, FSH consists of subunits designated as alpha and beta. Hormones of this type have alpha subunits that are very similar in structure, therefore the biological and immunological properties of each hormone are dependent on the unique beta subunit. In the female, FSH stimulates the growth and maturation of ovarian follicles by acting directly on the receptors located on the granulosa cells, follicular steroidogenesis is promoted and LH production is stimulated. The LH produced then binds to the theca cells and stimulates steroidogenesis. Increased intraovarian estradiol production occurs as follicular maturation advances, thereupon stimulating increased FSH receptor activity and FSH follicular binding. FSH, LH, and estradiol are therefore intimately related in supporting ovarian recruitment and maturation in women.FSH levels are elevated after menopause, castration, and in premature ovarian failure. The levels of FSH may be normalized through the administration of estrogens, which demonstrate a negative feedback mechanism. Abnormal relationships between FSH and LH, and between FSH and estrogen have been linked to anorexia nervosa and polycystic ovarian disease. Although there are significant exceptions, ovarian failure is indicated when random FSH concentrations exceed 40 mIU/mL. The growth of the seminiferous tubules and maintenance of spermatogenesis in men are regulated by FSH. However, androgens, unlike estrogens, do not lower FSH levels, therefore demonstrating a feedback relationship only with serum LH.

Pathways: Peptide Hormone Metabolism, Hormone Activity, C21-Steroid Hormone Metabolic Process