SFRP2 ELISA Kit

Catalog No. ABIN455159

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse SFRP2.

Quick Overview for SFRP2 ELISA Kit (ABIN455159)

Target: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details SFRP2 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of mouse Free-T3 concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse Free-T3.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 0.078 pmol/L
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Mus musculus,Mouse,Secreted frizzled-related protein 2,sFRP-2,Protein SDF5,Secreted apoptosis-related protein 1,SARP-1,Sfrp2,Sarp1,Sdf5

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Free-T3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Free-T3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Free-T3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Free-T3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for SFRP2

Target: SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))

Alternative Name: Sfrp2

Pathways: WNT Signaling, Tube Formation, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Positive Regulation of fat Cell Differentiation