APH1A ELISA Kit

Catalog No. ABIN455171

This Colorimetric ELISA kit is designed for the quantitative measurement of Human APH1A.

Quick Overview for APH1A ELISA Kit (ABIN455171)

Target: APH1A (Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Product Details APH1A ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of human gamma-secretase, ? -secretase/GACE concentrations in cell culture supernates, serum, plasma, tissue homogenates and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural human ? -secretase/GACE.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 0.39 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Homo sapiens,Human,Gamma-secretase subunit APH-1A,APH-1a,Aph-1alpha,Presenilin-stabilization factor,APH1A,PSF,CGI-78,UNQ579/PRO1141

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to γ -secretase/GACE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for γ -secretase/GACE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain γ -secretase/GACE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of γ -secretase/GACE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for APH1A

Target: APH1A (Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A))

Alternative Name: APH1A

Pathways: Notch Signaling, Neurotrophin Signaling Pathway