Haptoglobin ELISA Kit
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- Target See all Haptoglobin (HP) ELISA Kits
- Haptoglobin (HP)
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Reactivity
- Cow
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.78-50 μg/mL
- Minimum Detection Limit
- 0.78 μg/mL
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of bovine Hpt concentrations in serum, plasma and other biological fluids.
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay recognizes recombinant and natural bovine Hpt.
- Cross-Reactivity (Details)
- No significant cross-reactivity or interference was observed.
- Sensitivity
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< 0.195 μg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. - Characteristics
- Bos taurus,Bovine,Haptoglobin,HP,Zonulin
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- Discover our best selling HP ELISA Kit
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- Discover our top product HP ELISA Kit
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hpt. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hpt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hpt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Hpt in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
- Storage Comment
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Target See all Haptoglobin (HP) ELISA Kits
- Haptoglobin (HP)
- Alternative Name
- HP (HP Products)
- Synonyms
- HP ELISA Kit, wu:fb64e01 ELISA Kit, BP ELISA Kit, HP2ALPHA2 ELISA Kit, HPA1S ELISA Kit, HP-1 ELISA Kit, HPR ELISA Kit, Zonulin ELISA Kit, haptoglobin ELISA Kit, haptoglobin-like ELISA Kit, HP ELISA Kit, hp ELISA Kit, Hp ELISA Kit, LOC479668 ELISA Kit, LOC101102413 ELISA Kit
- Background
- Haptoglobin (Hpt) is a plasma protein with hemoglobin-binding capacity, and a plasma glycoproteins that form a stable complex with hemoglobin to aid the recycling of heme iron. It is a well-known marker of hemolysis. High haptoglobin level in plasma was associated with an increased cardiovascular risk in obese men, inflammation, atherosclerosis, and systemic sclerosis.
- Pathways
- Transition Metal Ion Homeostasis
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