Haptoglobin ELISA Kit

Catalog No. ABIN455286

The Cow Haptoglobin ELISA Kit (ABIN455286) is a Colorimetric ELISA Kit designed to quantify Cow Haptoglobin.

Quick Overview for Haptoglobin ELISA Kit (ABIN455286)

Target: Haptoglobin (HP)

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 μg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Haptoglobin ELISA Kit

Minimum Detection Limit: 0.78 μg/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of bovine Hpt concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural bovine Hpt.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 0.195 μg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Bos taurus,Bovine,Haptoglobin,HP,Zonulin

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hpt. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hpt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hpt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Hpt in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for Haptoglobin

Target: Haptoglobin (HP)

Alternative Name: HP

Background: Haptoglobin (Hpt) is a plasma protein with hemoglobin-binding capacity, and a plasma glycoproteins that form a stable complex with hemoglobin to aid the recycling of heme iron. It is a well-known marker of hemolysis. High haptoglobin level in plasma was associated with an increased cardiovascular risk in obese men, inflammation, atherosclerosis, and systemic sclerosis.

Pathways: Transition Metal Ion Homeostasis