IL-10 ELISA Kit

Catalog No. ABIN455521

Mouse IL-10 ELISA Kit, Colorimetric assay for quantification of Mouse IL-10, that has been mentioned in 1 publication.

Quick Overview for IL-10 ELISA Kit (ABIN455521)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details IL-10 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of mouse Interleukin 10,IL-10 concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse IL-10.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 7.8 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Mus musculus,Mouse,Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,Il10,Il-10

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

References for IL-10 ELISA Kit (ABIN455521)

Takano, Yamamoto, Tomita, Takashina, Yokoo, Matsuda, Takano, Hattori: "Successful treatment of acute lung injury with pitavastatin in septic mice: potential role of glucocorticoid receptor expression in alveolar macrophages." in: The Journal of pharmacology and experimental therapeutics, Vol. 336, Issue 2, pp. 381-90, (2011) (PubMed).

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: Il10

Background: Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 cytokine family. This family currently comprises IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. All known IL-10 family members are secreted -helical proteins. IL-10 is a secreted, possibly glycosylated, polypeptide with a molecular weight of 18 kDa. Based on human studies, IL-10 is likely to circulate as a nondisulfide-linked homodimer. IL-10 is synthesized as a 181 amino acid (aa) precursor with a 19 aa signal sequence and a 162 aa mature form. The mature segment has one potential N-linked glycosylation site plus four cysteines which likely form two intra-chain disulfide bridges. Mature rat IL-10 shows 76%, 73%, 72%, 80%, 86%, 85% and 76% aa identity to guinea pig, mouse, human, feline and equine IL-10, respectively. Upon activation, mammalian cells known to secrete IL-10 include NK cells, cytotoxic CD8+ T cells secreting Th2-like cytokines, CD4+CD45RA- (memory) Th1 and Th2 cells, macrophages, monocytes, CD5+ and CD5- B cells, dendritic cells, hepatic stellate (Ito) cells, keratinocytes, melanoma cells, mast cells, placental cytotrophoblasts, and fetal erythroblasts.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints