Interleukin 17a ELISA Kit

Catalog No. ABIN455540

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse Interleukin 17a.

Quick Overview for Interleukin 17a ELISA Kit (ABIN455540)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma

Product Details Interleukin 17a ELISA Kit

Purpose: This immunoassay kit allows for the specific measurement of mouse IL-17 concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse IL-17.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Characteristics: Mus musculus,Mouse,Interleukin-17A,IL-17,IL-17A,Cytotoxic T-lymphocyte-associated antigen 8,CTLA-8,Il17a,Ctla8,Il17

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for IL-17 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: Il17a

Background: Interleukin 17 (IL-17, also known as IL-17A) is a 30 - 35 kDa variably glycosylated homodimeric protein that belongs to a unique family of cysteine-knot related proteins. Its sequence was originally isolated from an activated rodent hybridoma and termed CTLA-8 . It is synthesized as a 155 amino acid (aa) precursor that contains a 23 aa signal sequence and a 15 kDa, 132 aa mature segment. Although there are two intrachain disulfide bonds that create a ring reminiscent of those found in cysteine-knot proteins, the actual closed knot structure does not appear to form. IL-17 has one potential N-linked glycosylation site. Mature rat IL-17 is 61% and 60% aa identical to mouse and rat IL-17, respectively. It also shows limited aa sequence identity to other rat IL-17 family members. In particular, it shows 34%, 38%, 34% and 26%aa sequence identity to IL-17B, C, D, and E, respectively, and 49% aa sequence identity to IL-17F with which it is most homologous. While rodent and rat mature sequences show modest aa sequence identity, rat IL-17 is active on both mouse and rat cells. The cells principally known to produce IL-17 are the memory CD4+ T cells. In addition, CD8+ T cells as well as TCR+ CD4-CD8- T cells, neutrophils (PMNs) and eosinophils have also been reported to express mRNA transcripts for IL-17.