HAVCR1 ELISA Kit

Catalog No. ABIN455655

The Mouse HAVCR1 ELISA Kit (ABIN455655) is a Colorimetric ELISA Kit designed to quantify Mouse HAVCR1. This ELISA Kit has been cited in 1 publication.

Quick Overview for HAVCR1 ELISA Kit (ABIN455655)

Target: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156-10 ng/mL

Application: ELISA

Sample Type: Serum, Urine, Plasma

Product Details HAVCR1 ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: This immunoassay kit allows for the specific measurement of Mouse KIM-1 concentrations in serum,urine and plasma.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural Mouse KIM-1.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Characteristics: Mus musculus,Mouse,Hepatitis A virus cellular receptor 1 homolog,HAVcr-1,Kidney injury molecule 1,KIM-1,T cell immunoglobulin and mucin domain-containing protein 1,TIMD-1,T cell membrane protein 1,TIM-1,

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for KIM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KIM-1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for KIM-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

References for HAVCR1 ELISA Kit (ABIN455655)

Gil, Yang, Lee, Hong: "Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication." in: Clinical toxicology (Philadelphia, Pa.), Vol. 47, Issue 9, pp. 870-5, (2009) (PubMed).

Target Details for HAVCR1

Target: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Alternative Name: Havcr1

Target Type: Virus

Background: Kidney injury molecule-1 (KIM-1) is a type 1 transmembrane protein that is not detectable in normal kidney tissue but is expressed at high levels in rodent kidneys with dedifferentiated proximal tubule epithelial cells after ischemic or toxic injury.