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Factor VIII ELISA Kit

F8 Reactivity: Human Colorimetric Sandwich ELISA 12.5-800 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN456987
  • Target See all Factor VIII (F8) ELISA Kits
    Factor VIII (F8) (Coagulation Factor VIII (F8))
    Reactivity
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    12.5-800 ng/mL
    Minimum Detection Limit
    12.5 ng/mL
    Application
    ELISA
    Sample Type
    Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay recognizes recombinant and natural human F 8 .
    Cross-Reactivity (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensitivity
    3.12 ng/mL
    Components
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
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  • Application Notes
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Comment

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Sample Volume
    100 μL
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to F 8 . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for F 8 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain F 8 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of F 8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
    Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
    • Intra-assay: CV% less than 8%
    • Inter-assay: CV% less than 10%
    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Handling Advice
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Storage
    4 °C/-20 °C
    Storage Comment
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Expiry Date
    6 months
  • Periayah, Halim, Mat Saad, Yaacob, Hussein, Abdul Karim, Abdul Rashid, Ujang: "Effect of the Novel Biodegradable N, O-Carboxymethylchitosan and Oligo-Chitosan on the Platelet Thrombogenicity Cascade in von Willebrand Disease." in: Thrombosis research, Vol. 136, Issue 3, pp. 625-33, (2015) (PubMed).

    Wang, Yuan, Zhong, Wen, Deng, Liang, Zheng: "The anticoagulant effect of PGI2S and tPA in transgenic umbilical vein endothelial cells is linked to up-regulation of PKA and PKC." in: International journal of molecular sciences, Vol. 15, Issue 2, pp. 2826-39, (2014) (PubMed).

  • Target See all Factor VIII (F8) ELISA Kits
    Factor VIII (F8) (Coagulation Factor VIII (F8))
    Alternative Name
    coagulation factor VIII (FVIII) (F8 Products)
    Synonyms
    fb61d02 ELISA Kit, wu:fb61d02 ELISA Kit, Cf-8 ELISA Kit, Cf8 ELISA Kit, FVIII ELISA Kit, AHF ELISA Kit, DXS1253E ELISA Kit, F8B ELISA Kit, F8C ELISA Kit, HEMA ELISA Kit, coagulation factor VIIi ELISA Kit, coagulation factor VIII ELISA Kit, coagulation factor VIII, procoagulant component ELISA Kit, f7i ELISA Kit, F8 ELISA Kit
    Background
    Synonyms: RP11-115M6.7, AHF, DXS1253E, F8B, F8C, FVIII, HEMA, coagulation factor VIII|coagulation factor VIIIc|factor VIII F8B
    UniProt
    P00451
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