IgG ELISA Kit
Quick Overview for IgG ELISA Kit (ABIN457295)
Target
See all IgG ELISA KitsReactivity
Detection Method
Method Type
Application
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Minimum Detection Limit
- 15.6 ng/mL
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Purpose
- The IgG(bovine)-ELISA is designed for the quantitative determination of bovine IgG in complex samples.
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Analytical Method
- Quantitative
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Specificity
- IgG (mouse) <0.1%
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Characteristics
- The determination of bovine IgG is carried out as direct sandwich ELISA. Main application of the assay is to monitor purification of protein preparations (e.g. monoclonal antibodies) if bovine or calf serum has been used in the production process.
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IgG ELISA Kit
Reactivity: Rabbit Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP Reactivity: Rabbit
Mouse TrueBlot® Set (with IP beads)WB, IP Reactivity: Mouse
IgG ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Mouse Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Human Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Chicken Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Cow Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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Plate
- Pre-coated
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Protocol
- An antibody specific for bovine IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
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Assay Procedure
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm -
Assay Precision
- Intraassay precision (CV): (n=12) at 35 ng/ml: 2.9% at 112 ng/ml: 5.4%
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- IgG
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Target Type
- Antibody
Target See all IgG ELISA Kits
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