EBV EA(D) IgM ELISA Kit

Catalog No. ABIN459356

This Colorimetric ELISA kit is designed for the quantitative measurement of Human EBV EA(D) IgM.

Quick Overview for EBV EA(D) IgM ELISA Kit (ABIN459356)

Target: EBV EA(D) IgM (Anti-Epstein-Barr Virus Early Antigen (D) IgM (EBV EA(D) IgM))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum

Product Details EBV EA(D) IgM ELISA Kit

Purpose: anti-EA (D) EBV IgM assay is a solid-phase immunoanalytical test.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips coated with recombinant antigen 1 microplate
• 1.3 mL Negative control serum, r.t.u.1) CTRL - 1 vial
• 1.3 mL Positive control serum r.t.u. CTRL + 1 vial
• 1.3 mL Calibrator, r.t.u. CAL 1 vial
• 13 mL Anti-human IgM antibodies labelled with horseradish peroxidase (Px-conjugate) r.t.u., CONJ 1 vial
• 55 mL Wash buffer concentrate, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 2.0 mL RF sorbent, 25x concentrated RF SORBENT 25x 1 vial
• 13 mL Chromogenic substrate r.t.u. TMB 1 vial
• 13 mL Stop solution, r.t.u. STOP 1 vial
• Sealable pouch for unused strips Instruction manual
• Certificate
• of quality 1) (ready to use) 1 Chromogenic substrate TMB is compatible and interchangeable between ELISA-VIDITEST kits which contain TMB and not with other Chromogenic substrates TMB-O, TMB-BF.

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-EA (D) EBV IgM assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a recombinant antigen that bears immunodominant epitopes of EA protein. The anti- EA (D) antibodies, if present in the serum (plasma), bind to the immobilized antigens. The antibodies that do not bind are washed away and those that formed complexes with the antigens are later on recognised by animal anti-human IgM antibodies labelled with h orseradish peroxidase. The presence of labelled antibodies is revealed by an enzymatic reaction with a chrom ogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed t o the reaction background. To eliminate a false positive results the test utilises the ""Dilution buffer plus"", which is prepared by mixing the standard Dilution buffer r.t.u. with RF sorbent according to the Instruction manual. Dilution buffer plus contains anti-Human IgG antibodies that eliminate binding of the rheum atoid factor (RF) (autoantibodies to the Fc portions of immunoglobulins) and decreases the interferenc e of the specific IgG antibodies.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit and the kit reagents at +2 to +10°C, in a dry place and protected from the light. Store unused strips in the sealable pouch and keep the desiccant inside. Store serum samples at +2 to +10°C up to one week. For longer period make aliquots and keep them at - 20°C. Avoid repeated thawing and freezing. Do not store diluted samples. Always prepare fresh. Kits are shipped in cooling bags, the transport time of 72 hours have no influence on expiration. If you find damage at any part of the kit, please inform the manufacturer. Expiration date is indicated at the ELISA kit label and at all reagent labels.

Target Details for EBV EA(D) IgM

Target: EBV EA(D) IgM (Anti-Epstein-Barr Virus Early Antigen (D) IgM (EBV EA(D) IgM))

Target Type: Antibody