EBV NA IgG ELISA Kit

Catalog No. ABIN459361

Human EBV NA IgG ELISA Kit, Colorimetric assay for quantification of Human EBV NA IgG.

Quick Overview for EBV NA IgG ELISA Kit (ABIN459361)

Target: EBV NA IgG (Anti-Epstein-Barr Virus Nuclear Antigen IgG (EBV NA IgG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Serum, Plasma

Product Details EBV NA IgG ELISA Kit

Purpose: anti-EBNA-1 EBV IgG is a solid-phase immunoanalytical test.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Standard A = Negative control human serum, r.t.u. 1) ST A/NC 1 vial
• 1.3 mL Standard B (human serum) r.t.u. ST B 1 vial
• 1.3 mL Standard C (human serum) r.t.u. ST C 1 vial
• 2.0 mL Standard D = Calibrator (human serum), r.t.u. ST D/CAL 1 vial
• 1.3 mL Standard E = Positive control human serum, r.t.u. ST E/PC 1 vial
• 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O 2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-EBNA-1 EBV IgG is a solid-phase immunoanalytical test. A specific recombinant antigen of EBNA-1 EBV is bound to the surface of the wells. If relevant antibodies are present in the test samples, they bind to the immobilized antigens. In the next step, the bound antibodies then react with horseradish peroxidase-labeled anti-human IgG antibodies. The amount of bound labeled antibodies is determined by a color enzymatic reaction. Negative samples do not react, a slight change in the color of the wells is the background of the reaction.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for EBV NA IgG

Target: EBV NA IgG (Anti-Epstein-Barr Virus Nuclear Antigen IgG (EBV NA IgG))

Target Type: Antibody