HSV1/2 IgG ELISA Kit

Catalog No. ABIN459368

This Colorimetric ELISA kit is designed for the quantitative measurement of Human HSV1/2 IgG.

Quick Overview for HSV1/2 IgG ELISA Kit (ABIN459368)

Target: HSV1/2 IgG (Anti-Herpes Simplex Virus Type 1/2 IgG (HSV1/2 IgG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Serum, Plasma

Product Details HSV1/2 IgG ELISA Kit

Purpose: anti-HSV 1+2 IgG is a solid-phase immunoanalytical test.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Standard A = Negative control human serum, r.t.u. 1) ST A/NC 1 vial
• 2.0 mL Standard D = Calibrator (human serum), r.t.u. ST D/CAL 1 vial
• 1.3 mL Standard E = Positive control human serum, r.t.u. ST E/PC 1 vial
• 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-HSV 1+2 IgG is a solid-phase immunoanalytical test. The polystyrene strips are coated with a mixture of antigens, which bear immunodominant epitopes of HSV 1 and HSV 2. Anti-HSV antibodies from samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away those that formed complexes with the antigens are later on recognized by animal anti-human IgG antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative samples do not react and the mild change in colour, if present, may be attributed to the reaction background.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for HSV1/2 IgG

Target: HSV1/2 IgG (Anti-Herpes Simplex Virus Type 1/2 IgG (HSV1/2 IgG))

Target Type: Antibody