EBV VCA IgA ELISA Kit

Catalog No. ABIN459374

This Colorimetric ELISA kit is designed for the quantitative measurement of Human EBV VCA IgA.

Quick Overview for EBV VCA IgA ELISA Kit (ABIN459374)

Target: EBV VCA IgA (Anti-Epstein-Barr Virus Viral Capsid Antigen IgA (EBV VCA IgA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Serum

Product Details EBV VCA IgA ELISA Kit

Purpose: anti-VCA EBV IgA is a solid-phase immunoanalytical test.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Components:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 2 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 13 mL Anti-human IgA animal antibodies labelled with horseradish peroxidase (anti-IgA Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H 2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-VCA EBV IgA is a solid-phase immunoanalytical test. A specific antigen representing immunodominant epitopes of the VCA complex is bound to the surface of the wells. If antibodies are present in the test samples, they will bind to the immobilized proteins. The bound antibodies then react in the next step with horseradish peroxidase-labeled anti-human IgA antibodies. The amount of bound labeled antibodies is determined by a color enzymatic reaction. Negative samples do not react, a slight change in the color of the wells is the background of the reaction.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for EBV VCA IgA

Target: EBV VCA IgA (Anti-Epstein-Barr Virus Viral Capsid Antigen IgA (EBV VCA IgA))

Target Type: Antibody