CAMP ELISA Kit

Catalog No. ABIN4881300

Product Details CAMP ELISA Kit

Target: CAMP (cAMP) (Cyclic Adenosine Monophosphate (cAMP))

Reactivity: Chemical

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.1-1.000 ng/mL

Minimum Detection Limit: 0.1 ng/mL

Application: ELISA

Purpose: cAMP EIA Kit optimized for serum and plasma samples. Competition-based ELISA on a 96-well strip plate.

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This EIA kit is designed to only detect Cyclic AMP.

Sensitivity: 4.4 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Standard, Biotinylated cAMP peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Expiry Date: 6 months

Target Details for CAMP

Target: CAMP (cAMP) (Cyclic Adenosine Monophosphate (cAMP))

Alternative Name: cAMP, Cyclic AMP (cAMP Products)

Synonyms: CRAMP ELISA Kit, CAP-18 ELISA Kit, CAP18 ELISA Kit, FALL-39 ELISA Kit, FALL39 ELISA Kit, LL37 ELISA Kit, CAP11 ELISA Kit, Cnlp ELISA Kit, Cramp ELISA Kit, MCLP ELISA Kit, CATHL7 ELISA Kit, cathelicidin antimicrobial peptide ELISA Kit, Camp ELISA Kit, CAMP ELISA Kit

Target Type: Chemical

Pathways: Cellular Response to Molecule of Bacterial Origin