Urocortin ELISA Kit

Catalog No. ABIN4881336

Mouse, Human and Rat Urocortin ELISA Kit, Colorimetric assay for quantification of Mouse, Human and Rat Urocortin.

Quick Overview for Urocortin ELISA Kit (ABIN4881336)

Target: Urocortin (UCN)

Reactivity: Mouse, Human, Rat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.1 ng/mL - 1000 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Urocortin ELISA Kit

Minimum Detection Limit: 0.1 ng/mL

Purpose: Human/Mouse/Rat Urocortin EIA Kit optimized for serum. Competition-based ELISA

Analytical Method: Quantitative

Specificity: This EIA kit is designed to detect human, mouse, and rat active Urocortin.

Sensitivity: 1.2 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Expiry Date: 6 months

Target Details for Urocortin

Target: Urocortin (UCN)

Alternative Name: Urocortin

Background:

Gene Symbols: UCN

Background: Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is an endogenous ligand for corticotropin-releasing factor (CRF) receptors. Urocortin, peptide of 40 amino acids, acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH). It binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin plays a role in the establishment of normal hearing thresholds. It reduces food intake and regulates ghrelin levels in gastric body and plasma.

Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow.

Protein Name (Synonyms): Urocortin

Gene ID: 7349

UniProt: P55089

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of Cell Size, Feeding Behaviour