×
For best experience we recommend to activate Javascript in your browser.
IGFBP2 ELISA Kit
IGFBP2
Reactivity: Dog
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details IGFBP2 ELISA Kit
(hide)
Target
See all IGFBP2 ELISA Kits
IGFBP2
(Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
Reactivity
All reactivities for IGFBP2 elisa kits
Dog
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Canine (dog) Insulin-like Growth Factor Binding Protein 2 (IGFBP2) ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes Canine Insulin-like Growth Factor Binding Protein 2 (IGFBP2)
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Featured
Discover our best selling IGFBP2 ELISA Kit
Top Product
Discover our top product IGFBP2 ELISA Kit
Alternatives
(show)
Application Details
(hide)
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
(hide)
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for IGFBP2
(hide)
Target
See all IGFBP2 ELISA Kits
IGFBP2
(Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
Alternative Name
IGFBP-2 (IGFBP2 Products )
Synonyms
IBP2 ELISA Kit, IGF-BP53 ELISA Kit, AI255832 ELISA Kit, IBP-2 ELISA Kit, Igfbp-2 ELISA Kit, mIGFBP-2 ELISA Kit, IGFBP-2 ELISA Kit, ILGFBPA ELISA Kit, pIGFBP-2 ELISA Kit, igfbp2 ELISA Kit, MGC65741 ELISA Kit, MGC76823 ELISA Kit, MGC174445 ELISA Kit, IGFBP2 ELISA Kit, ibp2 ELISA Kit, igf-bp53 ELISA Kit, igfbp2a ELISA Kit, si:ch211-2k18.3 ELISA Kit, insulin like growth factor binding protein 2 ELISA Kit, insulin-like growth factor binding protein 2 ELISA Kit, insulin-like growth factor binding protein 2a ELISA Kit, insulin-like growth factor binding protein 2b ELISA Kit, IGFBP2 ELISA Kit, Igfbp2 ELISA Kit, igfbp2a ELISA Kit, igfbp2 ELISA Kit, igfbp2b ELISA Kit
Pathways
Myometrial Relaxation and Contraction , Growth Factor Binding , Activated T Cell Proliferation
Recently viewed
(hide)