IGFBP2 ELISA Kit

Catalog No. ABIN4881520

This Colorimetric ELISA kit is designed for the quantitative measurement of Dog IGFBP2.

Quick Overview for IGFBP2 ELISA Kit (ABIN4881520)

Target: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Reactivity: Dog

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.055 ng/mL - 12.5 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details IGFBP2 ELISA Kit

Minimum Detection Limit: 0.055 ng/mL

Purpose: Canine IGFBP-2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects Canine IGFBP-2 and Human IGFBP-2. Other species not determined. This ELISA kit shows no cross-reactivity with the following cytokines tested: Canine ErbB3 (Her3), Galectin-3, GASP-1, KC (CXCL1), MMP-8, NCAM-1 (CD56), Nope, PDGF-BB, TGF-alpha.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μl to 1 μl volumes
  • Adjustable 1-25 μl pipettes for reagent preparation
  • 100 μl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
  ,
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μl to 1 μl volumes
    • Adjustable 1-25 μl pipettes for reagent preparation
    • 100 μl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 4000 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4^°C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: May be stored for up to 6 months at 2^° to 8^°C from the date of shipment. Standard (recombinant protein) should be stored at -20^°C or -80^°C (recommended at -80^°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2^° to 8^°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20^°C.

Expiry Date: 6 months

Target Details for IGFBP2

Target: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Alternative Name: IGFBP-2

Background:

Gene Symbols: IGFBP2

Protein Name (Synonyms): Insulin like growth factor binding protein 2

UniProt: F1PEM7

Pathways: Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation