CPB1 ELISA Kit

Catalog No. ABIN4882085

Product Details CPB1 ELISA Kit

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Carboxypeptidase B1 (CPB1) ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CPB1

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Alternative Name: Carboxypeptidase B1 (CPB1 Products)

Synonyms: wu:fb60d07 ELISA Kit, wu:fb69b08 ELISA Kit, CPB1 ELISA Kit, pCPB ELISA Kit, CPB ELISA Kit, PASP ELISA Kit, PCPB ELISA Kit, Cpb ELISA Kit, 0910001A18Rik ELISA Kit, 1810063F02Rik ELISA Kit, 2210008M23Rik ELISA Kit, AI504870 ELISA Kit, carboxypeptidase B1 (tissue) ELISA Kit, carboxypeptidase B1 ELISA Kit, carboxypeptidase B1 S homeolog ELISA Kit, carboxypeptidase B ELISA Kit, cpb1 ELISA Kit, CPB1 ELISA Kit, cpb1.S ELISA Kit, CpipJ_CPIJ010799 ELISA Kit, CpipJ_CPIJ010801 ELISA Kit, VDBG_02663 ELISA Kit, LOC100562609 ELISA Kit, Cpb1 ELISA Kit

Gene ID: 1360

UniProt: P15086