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CNTNAP2 ELISA Kit

CNTNAP2 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4882106
  • Target See all CNTNAP2 ELISA Kits
    CNTNAP2 (Contactin Associated Protein-Like 2 (CNTNAP2))
    Reactivity
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human Caspr2 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CNTNAP2 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all CNTNAP2 ELISA Kits
    CNTNAP2 (Contactin Associated Protein-Like 2 (CNTNAP2))
    Alternative Name
    Caspr2 (CNTNAP2 Products)
    Synonyms
    AUTS15 ELISA Kit, CASPR2 ELISA Kit, CDFE ELISA Kit, NRXN4 ELISA Kit, PTHSL1 ELISA Kit, 5430425M22Rik ELISA Kit, Caspr2 ELISA Kit, mKIAA0868 ELISA Kit, CASPR ELISA Kit, MGC145769 ELISA Kit, MGC147944 ELISA Kit, MGC147981 ELISA Kit, contactin associated protein like 2 ELISA Kit, contactin associated protein-like 2 ELISA Kit, contactin associated protein like 2b ELISA Kit, CNTNAP2 ELISA Kit, Cntnap2 ELISA Kit, cntnap2b ELISA Kit, cntnap2 ELISA Kit
    Gene ID
    26047
    UniProt
    Q9UHC6
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