CD99L2 ELISA Kit

Catalog No. ABIN4882274

The Human CD99L2 ELISA Kit (ABIN4882274) is a Colorimetric ELISA Kit designed to quantify Human CD99L2.

Quick Overview for CD99L2 ELISA Kit (ABIN4882274)

Target: CD99L2 (CD99 Molecule-Like 2 (CD99L2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.07 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details CD99L2 ELISA Kit

Minimum Detection Limit: 0.07 ng/mL

Purpose: Human CD99-L2 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adenosine Deaminase/ADA, AIF, AKR1C4 , ASAH2, BCL-2, BID, Calreticulin, Calreticulin-2, CD314 (NKG2D), CD39L3/ENTPD3, CD51, CDC25B, Cerberus 1, CHST2, Cochlin, CRELD2, DC-SIGNR/CD299, eNOS, ENPP2/Autotaxin, FABP4, Fc epsilon RI, FGF R5, GALNT2, GALNT3, GIF, GPR111, beta-Glucuronidase/GUSB, Inhibin A, LILRB4/CD85k/ILT3, Neuroglycan C/CSPG5, NKp46/NCR1, Neuronal Pentraxin R/NPTXR, ROR2, SCCA2, Siglec-2/CD22, SIRP alpha/CD172a, SorCS1, Trypsin 1/PRSS1, Trypsin 3/PRSS3.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 5 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CD99L2

Target: CD99L2 (CD99 Molecule-Like 2 (CD99L2))

Alternative Name: CD99-L2

Background:

Gene Symbols: CD99L2, MIC2L1, UNQ1964/PRO4486

Protein Name (Synonyms): CD99 antigen-like protein 2 (MIC2-like protein 1) (CD antigen CD99)

Gene ID: 83692

UniProt: Q8TCZ2