CHMP2B ELISA Kit

Catalog No. ABIN4882336

Product Details CHMP2B ELISA Kit

Target: CHMP2B (Charged Multivesicular Body Protein 2B (CHMP2B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human CHMP2B ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Kallikrein 1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CHMP2B

Target: CHMP2B (Charged Multivesicular Body Protein 2B (CHMP2B))

Alternative Name: CHMP2B (CHMP2B Products)

Synonyms: ALS17 ELISA Kit, CHMP2.5 ELISA Kit, DMT1 ELISA Kit, VPS2-2 ELISA Kit, VPS2B ELISA Kit, 1190006E07Rik ELISA Kit, RGD1306781 ELISA Kit, chmp2b ELISA Kit, chmp2bl ELISA Kit, zgc:77025 ELISA Kit, si:ch211-224m9.5 ELISA Kit, CHMP2B ELISA Kit, cg4618 ELISA Kit, chmp2.5 ELISA Kit, dmt1 ELISA Kit, vps2-2 ELISA Kit, vps2b ELISA Kit, CHMP2b-B ELISA Kit, chmp2b-a ELISA Kit, chmp2b-b ELISA Kit, charged multivesicular body protein 2B ELISA Kit, charged multivesicular body protein 2Bb ELISA Kit, charged multivesicular body protein 2Ba ELISA Kit, charged multivesicular body protein 2B pseudogene ELISA Kit, charged multivesicular body protein 2B S homeolog ELISA Kit, charged multivesicular body protein 2B L homeolog ELISA Kit, CHMP2B ELISA Kit, Chmp2b ELISA Kit, chmp2bb ELISA Kit, chmp2ba ELISA Kit, LOC747711 ELISA Kit, chmp2b.S ELISA Kit, chmp2b.L ELISA Kit

Gene ID: 25978

UniProt: Q9UQN3