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BMPER ELISA Kit
BMPER
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details BMPER ELISA Kit
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Target
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BMPER
(BMP Binding Endothelial Regulator (BMPER))
Reactivity
All reactivities for BMPER elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human Crossveinless-2 (CV-2/BMPER) ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Human Crossveinless-2. Other species not yet determined.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for BMPER
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Target
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BMPER
(BMP Binding Endothelial Regulator (BMPER))
Alternative Name
Crossveinless-2 (BMPER Products )
Synonyms
BMPER ELISA Kit, CG15671 ELISA Kit, CT35855 ELISA Kit, Cv 2 ELISA Kit, Cv-2 ELISA Kit, Cv2 ELISA Kit, Dmel\\CG15671 ELISA Kit, cv 2 ELISA Kit, CV2 ELISA Kit, cv-2 ELISA Kit, crim3 ELISA Kit, cv2 ELISA Kit, cvl2 ELISA Kit, id:ibd5071 ELISA Kit, CV-2 ELISA Kit, CRIM3 ELISA Kit, 3110056H04Rik ELISA Kit, Crim3 ELISA Kit, RGD1563373 ELISA Kit, BMP binding endothelial regulator ELISA Kit, crossveinless 2 ELISA Kit, BMP-binding endothelial regulator protein ELISA Kit, BMP binding endothelial regulator L homeolog ELISA Kit, BMP-binding endothelial regulator ELISA Kit, BMPER ELISA Kit, cv-2 ELISA Kit, bmper ELISA Kit, LOC100070275 ELISA Kit, cv2 ELISA Kit, bmper.L ELISA Kit, Bmper ELISA Kit
Gene ID
168667
UniProt
Q8N8U9
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