CLEC7A ELISA Kit

Catalog No. ABIN4882593

Human CLEC7A ELISA Kit, Colorimetric assay for quantification of Human CLEC7A.

Quick Overview for CLEC7A ELISA Kit (ABIN4882593)

Target: CLEC7A (C-Type Lectin Domain Family 7, Member A (CLEC7A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.082 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details CLEC7A ELISA Kit

Minimum Detection Limit: 0.082 ng/mL

Purpose: Human Dectin-1 (CLEC7A) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human AMIGO, Aminopeptidase LRAP, Amnionless, Arylsulfatase A, Bcl-w, CD109, CD157, CD34, CD83, CLEC-1, CLEC10A, CMG-2, CREG, Cystatin SN, Cytokeratin-8, Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin, Lumican, Matriptase, MEP1B, Nectin-3, OX40, OX40 Ligand, p27, Pappalysin-2, Plexin B3, Plexin D1, proGRP, PSA-total, Reg1B, RGM-A, ROBO2, Spinesin, TWEAK R, ULBP-3.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 2 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details for CLEC7A

Target: CLEC7A (C-Type Lectin Domain Family 7, Member A (CLEC7A))

Alternative Name: Dectin-1

Background:

Gene Symbols: CLEC7A, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082

Protein Name (Synonyms): C-type lectin domain family 7 member A (Beta-glucan receptor) (C-type lectin superfamily member 12) (Dendritic cell-associated C-type lectin 1) (DC-associated C-type lectin 1) (Dectin-1)

Gene ID: 64581

UniProt: Q9BXN2

Pathways: Activation of Innate immune Response