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DPPA4 ELISA Kit

DPPA4 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4882637
$764.50
Plus shipping costs $45.00
Shipping to: United States
Delivery in 31 to 41 Business Days
  • Target See all DPPA4 ELISA Kits
    DPPA4 (Developmental Pluripotency Associated 4 (DPPA4))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human DPPA4 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all DPPA4 ELISA Kits
    DPPA4 (Developmental Pluripotency Associated 4 (DPPA4))
    Alternative Name
    DPPA4 (DPPA4 Products)
    Synonyms
    2410091M23Rik ELISA Kit, C76608 ELISA Kit, ECAT15-1 ELISA Kit, DPPA4 ELISA Kit, developmental pluripotency associated 4 ELISA Kit, DppA4 ELISA Kit, peptide ABC transporter substrate-binding protein ELISA Kit, dipeptide ABC transport system, substrate-binding protein ELISA Kit, DPPA4 ELISA Kit, Dppa4 ELISA Kit, dppA4 ELISA Kit, HVO_RS14920 ELISA Kit, LOC100360556 ELISA Kit
    Gene ID
    55211
    UniProt
    Q7L190
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