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DPPA4 ELISA Kit
DPPA4
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4882637
$764.50
Plus shipping costs $45.00
96 tests ABIN4882637
2 x 96 tests ABIN4882638
5 x 96 tests ABIN4882639
96 tests ABIN4882637
2 x 96 tests ABIN4882638
5 x 96 tests ABIN4882639
Delivery in 31 to 41 Business Days
Product Details DPPA4 ELISA Kit
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Target
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DPPA4
(Developmental Pluripotency Associated 4 (DPPA4))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human DPPA4 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for DPPA4
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Target
See all DPPA4 ELISA Kits
DPPA4
(Developmental Pluripotency Associated 4 (DPPA4))
Alternative Name
DPPA4 (DPPA4 Products )
Synonyms
2410091M23Rik ELISA Kit, C76608 ELISA Kit, ECAT15-1 ELISA Kit, DPPA4 ELISA Kit, developmental pluripotency associated 4 ELISA Kit, DppA4 ELISA Kit, peptide ABC transporter substrate-binding protein ELISA Kit, dipeptide ABC transport system, substrate-binding protein ELISA Kit, DPPA4 ELISA Kit, Dppa4 ELISA Kit, dppA4 ELISA Kit, HVO_RS14920 ELISA Kit, LOC100360556 ELISA Kit
Gene ID
55211
UniProt
Q7L190