FOXP3 ELISA Kit
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- Target See all FOXP3 ELISA Kits
- FOXP3 (Forkhead Box P3 (FOXP3))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Custom Human FoxP3 ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair recognizes .
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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- Target See all FOXP3 ELISA Kits
- FOXP3 (Forkhead Box P3 (FOXP3))
- Alternative Name
- FoxP3 (FOXP3 Products)
- Synonyms
- foxp3 ELISA Kit, FOXP3 ELISA Kit, AIID ELISA Kit, DIETER ELISA Kit, IPEX ELISA Kit, PIDX ELISA Kit, XPID ELISA Kit, JM2 ELISA Kit, scurfin ELISA Kit, sf ELISA Kit, RGD1562112 ELISA Kit, forkhead box P3 ELISA Kit, forkhead box P3a ELISA Kit, forkhead box P3 L homeolog ELISA Kit, FOXP3 ELISA Kit, foxp3a ELISA Kit, foxp3 ELISA Kit, foxp3.L ELISA Kit, Foxp3 ELISA Kit
- Gene ID
- 50943
- UniProt
- Q9BZS1
- Pathways
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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