GPR56 ELISA Kit

Catalog No. ABIN4883128

This Colorimetric ELISA kit is designed for the quantitative measurement of Human GPR56.

Quick Overview for GPR56 ELISA Kit (ABIN4883128)

Target: GPR56 (G Protein-Coupled Receptor 56 (GPR56))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 16 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details GPR56 ELISA Kit

Minimum Detection Limit: 16 pg/mL

Purpose: Human GPR56 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand (TNFSF9), Activin RIIB, Aminopeptidase P2 (XPNPEP2), BAMBI (NMA), BOC, Brevican, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320 (TCblR, 8D6A), CDNF, CDO, CHST1, CHST4, CILP-1, CNTF R alpha, CRIM1, CRTAC1, CXADR, Dopa Decarboxylase (DDC), DPPII (QPP, DPP7), DSPG3, EMR2, FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Gas6, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, Kirrel3 (NEPH2), KLF4, LAIR1, LAMP, LAMP1 (CD107a), MDGA1, MIS RII, Neurexin 3.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 2 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for GPR56

Target: GPR56 (G Protein-Coupled Receptor 56 (GPR56))

Alternative Name: GPR56

Background:

Gene Symbols: GPR56, ADGRG1, TM7LN4, TM7XN1, UNQ540/PRO1083

Protein Name (Synonyms): Adhesion G-protein coupled receptor G1 (G-protein coupled receptor 56) (Protein TM7XN1) [Cleaved into: ADGRG1 N-terminal fragment (ADGRG1 NT) (GPR56 N-terminal fragment) (GPR56 NT) (GPR56(N)) (GPR56 extracellular subunit) (GPR56 subunit alpha) ADGRG1 C-terminal fragment (ADGRG1 CT) (GPR56 C-terminal fragment) (GPR56 CT) (GPR56(C)) (GPR56 seven-transmembrane subunit) (GPR56 7TM) (GPR56 subunit beta)

Gene ID: 9289

UniProt: Q9Y653