HMGB3 ELISA Kit

Catalog No. ABIN4883200

Product Details HMGB3 ELISA Kit

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human HMGB3 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for HMGB3

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Alternative Name: HMGB3 (HMGB3 Products)

Synonyms: HMG-2a ELISA Kit, HMG-4 ELISA Kit, HMG2A ELISA Kit, HMG4 ELISA Kit, Hmg2a ELISA Kit, Hmg4 ELISA Kit, RGD1564407 ELISA Kit, hmgb3 ELISA Kit, MGC54022 ELISA Kit, Xhmgb3 ELISA Kit, MGC88931 ELISA Kit, fa19b06 ELISA Kit, fj43d02 ELISA Kit, wu:fa19b06 ELISA Kit, wu:fj43d02 ELISA Kit, zgc:112073 ELISA Kit, HMGB3 ELISA Kit, NFD03 ELISA Kit, NFD3 ELISA Kit, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR ELISA Kit, high mobility group B3 ELISA Kit, HMG-1 ELISA Kit, HMG2a ELISA Kit, HMGB1 ELISA Kit, high mobility group box 3 ELISA Kit, high mobility group box 3b ELISA Kit, high mobility group box 3 S homeolog ELISA Kit, high mobility group protein ELISA Kit, high mobility group B3 ELISA Kit, High mobility group protein B3 ELISA Kit, HMGB3 ELISA Kit, Hmgb3 ELISA Kit, hmgb3 ELISA Kit, hmgb3b ELISA Kit, hmgb3.S ELISA Kit

Gene ID: 3149

UniProt: O15347