PSIP1 ELISA Kit

Catalog No. ABIN4883589

Product Details PSIP1 ELISA Kit

Target: PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human LEDGF ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for PSIP1

Target: PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))

Alternative Name: LEDGF (PSIP1 Products)

Synonyms: PSIP1 ELISA Kit, im:7149861 ELISA Kit, zgc:109898 ELISA Kit, LEDGF ELISA Kit, p75 ELISA Kit, PSIP2 ELISA Kit, DFS70 ELISA Kit, PAIP ELISA Kit, p52 ELISA Kit, AA408851 ELISA Kit, AU015605 ELISA Kit, Dfs70 ELISA Kit, Ledgfa ELISA Kit, Ledgfb ELISA Kit, Psip2 ELISA Kit, Ledgf ELISA Kit, SBP75 ELISA Kit, LEDGF/p75 ELISA Kit, PC4 and SFRS1 interacting protein 1 S homeolog ELISA Kit, PC4 and SFRS1 interacting protein 1 ELISA Kit, PC4 and SFRS1 interacting protein 1a ELISA Kit, LEDGF/p75 transcription factor-like ELISA Kit, psip1.S ELISA Kit, PSIP1 ELISA Kit, psip1a ELISA Kit, psip1 ELISA Kit, Psip1 ELISA Kit

Gene ID: 11168

UniProt: O75475

Pathways: Chromatin Binding, Ribonucleoprotein Complex Subunit Organization