Lactoferrin ELISA Kit

Catalog No. ABIN4883682

Product Details Lactoferrin ELISA Kit

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.96-480 ng/mL

Minimum Detection Limit: 1.96 ng/mL

Application: ELISA

Purpose: Human Lactoferrin ELISA Kit for serum, plasma, and cell culture supernatant samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adipsin, Angiogenin, B2M, bIG-H3, CD14, CRP, DPPIV, Endostatin, E-Selectin, FAP, Ferritin, Fetuin A, gp130, HGFR, ICAM-2, IGFBP-2, IGFBP-3, IGFBP-6, Lipocalin-2, L-Selectin, LYVE-1, MCSF R, MMP-9, NCAM-1, Nidogen-1, PAI-1, PF4, RBP4, SAA, TSP-1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: Lactoferrin (LTF Products)

Synonyms: LTF ELISA Kit, GIG12 ELISA Kit, HLF2 ELISA Kit, LF ELISA Kit, Lf ELISA Kit, Csp82 ELISA Kit, MMS10R ELISA Kit, Ms10r ELISA Kit, PLF ELISA Kit, TFEW ELISA Kit, conalbumin ELISA Kit, lactotransferrin ELISA Kit, transferrin ELISA Kit, transferrin (ovotransferrin) ELISA Kit, LTF ELISA Kit, CpipJ_CPIJ000114 ELISA Kit, TF ELISA Kit, Ltf ELISA Kit

Gene ID: 4057

UniProt: P02788

Pathways: Transition Metal Ion Homeostasis