NID2 ELISA Kit

Catalog No. ABIN4883922

Human NID2 ELISA Kit, Colorimetric assay for quantification of Human NID2.

Quick Overview for NID2 ELISA Kit (ABIN4883922)

Target: NID2 (Nidogen 2 (NID2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 49 pg/mL - 12000 pg/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details NID2 ELISA Kit

Minimum Detection Limit: 49 pg/mL

Purpose: Human Nidogen-2 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE, ALK-4, ADAM23, Artemin, CT-1, Cathepsin V, FABP1, FGF-20, GDF-8, HAI-1, IL-27 R alpha (WSX-1), Insulin R (CD220), Kallikrein 7, LIF R alpha, Lipocalin-1, LTbR (Lymphotoxin beta R), Mesothelin, MFRP, Neuropilin-2, Neurturin, Olfactomedin-2 (Noelin-2), p53, PDECGF, PDGF-CC, Progranulin, Ret, ROBO4, Semaphorin 6B, Serpin F1 (PEDF), SREC-I (SCARF1), SREC-II (SCARF2), TLR1, TLR3, TPP1, TREM-2, TrkC, TROY, Uromodulin, XIAP.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 8 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details for NID2

Target: NID2 (Nidogen 2 (NID2))

Alternative Name: Nidogen-2

Background:

Gene Symbols: NID2

Protein Name (Synonyms): Nidogen-2 (NID-2) (Osteonidogen)

Gene ID: 22795

UniProt: Q14112