NQO1 ELISA Kit

Catalog No. ABIN4883977

Product Details NQO1 ELISA Kit

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human NQO-1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for NQO1

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Alternative Name: NQO-1 (NQO1 Products)

Synonyms: zgc:77191 ELISA Kit, wu:fb63c10 ELISA Kit, nqo1 ELISA Kit, DHQU ELISA Kit, DIA4 ELISA Kit, DTD ELISA Kit, NMOR1 ELISA Kit, NMORI ELISA Kit, QR1 ELISA Kit, Dia4 ELISA Kit, AV001255 ELISA Kit, Dtd ELISA Kit, Nmo-1 ELISA Kit, Nmo1 ELISA Kit, Nmor1 ELISA Kit, Ox-1 ELISA Kit, Ox1 ELISA Kit, Qr1 ELISA Kit, NADPH-d ELISA Kit, NAD(P)H dehydrogenase, quinone 1 ELISA Kit, NAD(P)H quinone dehydrogenase 1 ELISA Kit, NAD(P)H dehydrogenase, quinone 1 L homeolog ELISA Kit, nqo1 ELISA Kit, NQO1 ELISA Kit, Bpet2092 ELISA Kit, nqo1.L ELISA Kit, Nqo1 ELISA Kit

Gene ID: 1728

UniProt: P15559