REG3A ELISA Kit

Catalog No. ABIN4884333

Human REG3A ELISA Kit, Colorimetric assay for quantification of Human REG3A.

Quick Overview for REG3A ELISA Kit (ABIN4884333)

Target: REG3A (Regenerating Islet-Derived 3 alpha (REG3A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.12 ng/mL - 25 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details REG3A ELISA Kit

Minimum Detection Limit: 0.12 ng/mL

Purpose: Human Reg3A ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM22, Arylsulfatase B/ARSB, B3GNT2, Carbonic Anhydrase VB, Caspase 7, Caspase 8, Integrin alpha M/CD11b, SIRP gamma/CD172g, CD39L2/ENTPD6, CD39L4/ENTPD5, Integrin alpha 2/CD49b, CD7, CEACAM3, Carboxypeptidase E/CPE, FABP6, FAM3C, GDF-3, GSTM1, Kallikrein 11, Kallikrein 12, Kremen-2, OSCAR, PTP1B, R-Spondin 2, S100A13, Semaphorin 4C, Sirtuin 1/SIRT1, SMPD1, Sortilin, SPINK1, Stabilin-2, SULT2A1, Transcobalamin II, THSD1, TrkA, UCH-L3, VAPA, vWF-A2, Wnt-4.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 4 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for REG3A

Target: REG3A (Regenerating Islet-Derived 3 alpha (REG3A))

Alternative Name: Reg3A

Background: Gene Symbols: Regenerating islet-derived protein 3-alpha (REG-3-alpha) (Hepatointestinal pancreatic protein) (HIP/PAP) (Human proislet peptide) (Pancreatitis-associated protein 1) (Regenerating islet-derived protein III-alpha) (Reg III-alpha) [Cleaved into: Regenerating islet-derived protein 3-alpha 16.5 kDa fo

Gene ID: 5068

UniProt: Q06141