S100A9 ELISA Kit

Catalog No. ABIN4884409

Product Details S100A9 ELISA Kit

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human S100 A9 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100 A9 (S100A9 Products)

Synonyms: 60B8AG ELISA Kit, CAGB ELISA Kit, CFAG ELISA Kit, CGLB ELISA Kit, L1AG ELISA Kit, LIAG ELISA Kit, MAC387 ELISA Kit, MIF ELISA Kit, MRP14 ELISA Kit, NIF ELISA Kit, P14 ELISA Kit, Mrp14 ELISA Kit, S100A9 ELISA Kit, MRP-14 ELISA Kit, p14 ELISA Kit, 60B8Ag ELISA Kit, AW546964 ELISA Kit, BEE22 ELISA Kit, Cagb ELISA Kit, GAGB ELISA Kit, L1Ag ELISA Kit, S100 calcium binding protein A9 ELISA Kit, S100 calcium binding protein A9 (calgranulin B) ELISA Kit, S100A9 ELISA Kit, S100a9 ELISA Kit

Gene ID: 6280

UniProt: P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins