Stabilin 2 (STAB2) ELISA Kit

Catalog No. ABIN4884674

The Human Stabilin 2 ELISA Kit (ABIN4884674) is a Colorimetric ELISA Kit designed to quantify Human Stabilin 2.

Quick Overview for Stabilin 2 (STAB2) ELISA Kit (ABIN4884674)

Target: Stabilin 2 (STAB2)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.96 ng/mL - 300 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details

Minimum Detection Limit: 0.96 ng/mL

Purpose: Human Stabilin-2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM22, Arylsulfatase B/ARSB, B3GNT2, Carbonic Anhydrase VB, Caspase 7, Caspase 8, Integrin alpha M/CD11b, SIRP gamma/CD172g, CD39L2/ENTPD6, CD39L4/ENTPD5, Integrin alpha 2/CD49b, CD7, CEACAM3, Carboxypeptidase E/CPE, FABP6, FAM3C, GDF-3, GSTM1, Kallikrein 11, Kallikrein 12, Kremen-2, OSCAR, PTP1B, Reg3A, R-Spondin 2, S100A13, Semaphorin 4C, Sirtuin 1/SIRT1, SMPD1, Sortilin, SPINK1, SULT2A1, Transcobalamin II, THSD1, TrkA, UCH-L3, VAPA, vWF-A2, Wnt-4.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 2 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details

Target: Stabilin 2 (STAB2)

Alternative Name: Stabilin-2

Background:

Gene Symbols: STAB2, FEEL2, FELL, FEX2, HARE

Protein Name (Synonyms): Stabilin-2 (FAS1 EGF-like and X-link domain-containing adhesion molecule 2) (Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavenger receptor 2) (FEEL-2) (Hyaluronan receptor for endocytosis) [Cleaved into: 190 kDa form stabilin-2 (190 kDa hyaluronan receptor for endocytosis)

Gene ID: 55576

UniProt: Q8WWQ8

Pathways: Glycosaminoglycan Metabolic Process