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Dual Specificity Phosphatase 3 (DUSP3) ELISA Kit

DUSP3 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4885040
  • Target See all Dual Specificity Phosphatase 3 (DUSP3) ELISA Kits
    Dual Specificity Phosphatase 3 (DUSP3)
    Reactivity
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human VHR ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes .
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product DUSP3 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all Dual Specificity Phosphatase 3 (DUSP3) ELISA Kits
    Dual Specificity Phosphatase 3 (DUSP3)
    Alternative Name
    VHR (DUSP3 Products)
    Synonyms
    dusp3 ELISA Kit, si:bz18k17.3 ELISA Kit, si:rp71-18k17.3 ELISA Kit, VHR ELISA Kit, RGD1560049 ELISA Kit, 2210015O03Rik ELISA Kit, 5031436O03Rik ELISA Kit, T-DSP11 ELISA Kit, dual specificity phosphatase 3 ELISA Kit, dual specificity phosphatase 3b ELISA Kit, dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related) ELISA Kit, DUSP3 ELISA Kit, dusp3b ELISA Kit, Dusp3 ELISA Kit
    Target Type
    Viral Protein
    Gene ID
    1845
    UniProt
    P51452
    Pathways
    Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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