DKK3 ELISA Kit

Details for Product No. ABIN4885192
Antigen
  • REIC
  • RIG
  • reic
  • dkk-3
  • AW061014
  • C87148
  • Reic
  • p29
  • dickkopf WNT signaling pathway inhibitor 3
  • dickkopf WNT signaling pathway inhibitor 3 S homeolog
  • DKK3
  • dkk3
  • dkk3.S
  • Dkk3
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
5
5
4
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Purpose Custom Human DKK-3 SpeedELISA Kit.
Brand SpeedELISA
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Characteristics
  • Compressed workflow with only one wash step and three incubation steps
  • 3 hours total processing time
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Streptavidin-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Capture Antibody
  • HRP Conjugated Detection Antibody
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Antigen
Alternative Name DKK-3 ( ELISA Kit Abstract)
Gene ID 27122
UniProt Q9UBP4
Sample Volume 100 μL
Assay Time 3 h
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 70 μL of the Antibody Cocktail to each well.
  3. Incubate 10 minutes at RT.
  4. Add 50 μL of sample or standard to each well.
  5. Incubate 2 h at RT.
  6. Wash Wells
  7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  8. Incubate 30 min at RT.
  9. Add 50 μL of Stop Solution to each well.
  10. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Expiry Date 6 months
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