HSP70 ELISA Kit

Catalog No. ABIN4885543

Product Details HSP70 ELISA Kit

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Mouse Heat Shock Protein 70 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Mouse Heat Shock Protein 70.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70 (HSP70 Products)

Synonyms: APG-2 ELISA Kit, HS24/P52 ELISA Kit, HSPH2 ELISA Kit, RY ELISA Kit, hsp70 ELISA Kit, hsp70RY ELISA Kit, CG31354 ELISA Kit, HSP70 ELISA Kit, Hsp70Bb ELISA Kit, hsp70B ELISA Kit, hsp70Bb-prime ELISA Kit, DmelCG5834 ELISA Kit, CG5834 ELISA Kit, HSPA1 ELISA Kit, HSP70B' ELISA Kit, HSPA6 ELISA Kit, ARABIDOPSIS HEAT SHOCK PROTEIN 70 ELISA Kit, ATHSP70 ELISA Kit, heat shock protein 70 ELISA Kit, LOC100305036 ELISA Kit, hsc70 ELISA Kit, Hsp70 ELISA Kit, Hsp70-1 ELISA Kit, Hsp70.1 ELISA Kit, hsp68 ELISA Kit, Hsp110 ELISA Kit, irp94 ELISA Kit, HSP70-2 ELISA Kit, HSPA1B ELISA Kit, HSPA2 ELISA Kit, hsp70-5 ELISA Kit, HSP70-1 ELISA Kit, HSP70.1 ELISA Kit, HSP70.2 ELISA Kit, heat shock protein family A (Hsp70) member 4 ELISA Kit, CG5834 gene product from transcript CG5834-RA ELISA Kit, heat shock protein 70 ELISA Kit, heat shock protein family A (Hsp70) member 6 ELISA Kit, heat shock 70kDa protein 2 ELISA Kit, heat shock 70 kD protein cognate ELISA Kit, Hsp70 family chaperone ELISA Kit, Heat shock protein 70 ELISA Kit, Heat shock protein 70, putative ELISA Kit, heat shock protein 1B ELISA Kit, heat shock protein family A member 4 ELISA Kit, heat shock 70kDa protein 1A ELISA Kit, heat shock protein 1 ELISA Kit, Heat Shock Protein ELISA Kit, heat shock cognate 70-kd protein ELISA Kit, Heat shock 70 kDa protein 1A ELISA Kit, HSPA4 ELISA Kit, Hsp70Bbb ELISA Kit, HSP70 ELISA Kit, HSPA6 ELISA Kit, HSPA2 ELISA Kit, PCC7424_2419 ELISA Kit, Isop_1041 ELISA Kit, CGB_C3390W ELISA Kit, Bacsa_1698 ELISA Kit, dnaK-B ELISA Kit, LOC100305036 ELISA Kit, Hspa1b ELISA Kit, Hspa4 ELISA Kit, HSPA1A ELISA Kit, hsp1 ELISA Kit, hsp-70 ELISA Kit, hsp70 ELISA Kit, LOC108348108 ELISA Kit

Gene ID: 15525

UniProt: Q3U2G2