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PKMYT1 ELISA Kit

PKMYT1 Reactivity: Human pThr495 Colorimetric Sandwich ELISA Cell Culture Lysate
Catalog No. ABIN4886252
  • Target See all PKMYT1 ELISA Kits
    PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))
    Binding Specificity
    pThr495, total
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-PKMYT1 (Thr495) and Total PKMYT1 ELISA Kit. This assay semi-quantitatively measures phophorylated PKMYT1 (Thr495) and Total PKMYT1 in lysate samples.
    Sample Type
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    This ELISA kit recognizes Human PKMYT1 phosphorylated at site Threonine-495 as well as total PKMYT1.
    Characteristics
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all PKMYT1 ELISA Kits
    PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))
    Alternative Name
    PKMYT1 (PKMYT1 Products)
    Synonyms
    MYT1 ELISA Kit, 6230424P17 ELISA Kit, AW209059 ELISA Kit, Myt1 ELISA Kit, RGD1305434 ELISA Kit, myt1 ELISA Kit, protein kinase, membrane associated tyrosine/threonine 1 ELISA Kit, protein kinase, membrane associated tyrosine/threonine 1 S homeolog ELISA Kit, PKMYT1 ELISA Kit, pkmyt1 ELISA Kit, Pkmyt1 ELISA Kit, pkmyt1.S ELISA Kit
    Gene ID
    9088
    UniProt
    Q99640
    Pathways
    Mitotic G1-G1/S Phases, M Phase
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