Caspase 3 ELISA Kit

Catalog No. ABIN4886357

This Human Caspase 3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Caspase 3. This ELISA Kit has been cited in1 publication.

Quick Overview for Caspase 3 ELISA Kit (ABIN4886357)

Target: Caspase 3 (CASP3)

Binding Specificity: Cleaved-Asp175, Uncleaved

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details Caspase 3 ELISA Kit

Purpose: CASP-3 (D175) ELISA Kit. This ELISA is for measuring cleaved CASP-3 (Asp-175) as well as CASP-3 in human cell lysates.

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human/mouse cleaved-caspase-3 cleaved at site Aspartic Acid-175 as well as caspase-3.

Characteristics:

• Rapidly measures cleaved protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-cleaved Antibody
• Anti-pan Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at -20°C. Item D, store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C.

Expiry Date: 6 months

References for Caspase 3 ELISA Kit (ABIN4886357)

Sekar, Omran, Gopalakrishnan, Howland, Snutch, Taghibiglou: "Elevated sterol regulatory elementary binding protein 1 and GluA2 levels in the hippocampal nuclear fraction of Genetic Absence Epilepsy Rats from Strasbourg." in: Epilepsy research, Vol. 136, pp. 1-4, (2018) (PubMed).

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation