Caspase 7 ELISA Kit

Catalog No. ABIN4889767

Product Details Caspase 7 ELISA Kit

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Binding Specificity: Cleaved-Asp198, Uncleaved

Reactivity: Human, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human/Mouse CASP-7 (D198) ELISA Kit. This ELISA is for measuring cleaved CASP-7 (Asp-198) as well as CASP-7 in human and mouse cell lysates.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human/mouse cleaved-caspase-7 cleaved at site Aspartic Acid-198 as well as caspase-7.

Characteristics:

• Simultaneously measure cleaved protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-cleaved Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Alternative Name: Caspase-7 (CASP7 Products)

Synonyms: xCaspase-7 ELISA Kit, caspase-7 ELISA Kit, caspase7 ELISA Kit, cmh-1 ELISA Kit, ice-lap3 ELISA Kit, mch3 ELISA Kit, CASP7 ELISA Kit, AI314680 ELISA Kit, CMH-1 ELISA Kit, ICE-IAP3 ELISA Kit, Mch3 ELISA Kit, mCASP-7 ELISA Kit, CASP-7 ELISA Kit, ICE-LAP3 ELISA Kit, LICE2 ELISA Kit, MCH3 ELISA Kit, Caspase-7 ELISA Kit, MCH3/SCA-2 ELISA Kit, SCA-2 ELISA Kit, caspase 7 ELISA Kit, caspase-7 ELISA Kit, caspase 7 S homeolog ELISA Kit, CASP7 ELISA Kit, xcaspase-7 ELISA Kit, casp7 ELISA Kit, casp7.S ELISA Kit, Casp7 ELISA Kit

UniProt: P55210

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity