Hydroxylated HIF-1 alpha (P402) ELISA Kit

Details for Product No. ABIN4889777, Supplier: Log in to see
Antigen
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
1
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Purpose Human Hydroxylated-HIF-1 alpha (P402) ELISA Kit. This ELISA is for measuring hydroxylated HIF-1 alpha (Pro-402) in human cell lysates.
Sample Type Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity The antibody pair provided in this kit recognizes human Hydroxylated-HIF-1alpha hydroxylated at site Proline-402
Characteristics
  • Rapidly measure hydroxylared protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-hydroxylated Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Antigen
UniProt Q16665
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
Supplier Images
ELISA image for Hydroxylated HIF-1 alpha (P402) ELISA Kit (ABIN4889777) HeLa cells were treated with MG132. Solubilize cells at 4 x 10^7 cells/ml in Cell Lys...
ELISA image for Hydroxylated HIF-1 alpha (P402) ELISA Kit (ABIN4889777) HeLa cells were treated or untreated with MG132 and DMOG. Cell lysates were analyzed ...
ELISA image for Hydroxylated HIF-1 alpha (P402) ELISA Kit (ABIN4889777) HeLa cells were treated or untreated with MG132 and DMOG. Cell lysates were analyzed ...
Did you look for something else?