GLP-1 ELISA Kit

Catalog No. ABIN4947610

The Human, Rat, Mouse, Dog, Cow and Rabbit GLP-1 ELISA Kit (ABIN4947610) is a Colorimetric ELISA Kit designed to quantify Human, Rat, Mouse, Dog, Cow and Rabbit GLP-1. This ELISA Kit has been cited in 15 publications.

Quick Overview for GLP-1 ELISA Kit (ABIN4947610)

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Rabbit

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 12.35 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details GLP-1 ELISA Kit

Minimum Detection Limit: 12.35 pg/mL

Purpose: ELISA Kit for Glucagon Like Peptide 1 (GLP1)

Analytical Method: Quantitative

Specificity:

This assay has high sensitivity and excellent specificity for detection of Glucagon Like Peptide 1 (GLP1).
No significant cross-reactivity or interference between Glucagon Like Peptide 1 (GLP1) and analogues was observed.

Sensitivity: < 4.26 pg/mL

Components:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Material not included:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Application Details

Application Notes:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Comment:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Sample Volume: 50 μL

Assay Time: 2 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Assay Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucagon Like Peptide 1 (GLP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucagon Like Peptide 1 (GLP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handling Advice: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage: 4 °C

Storage Comment:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Expiry Date: 6 months

References for GLP-1 ELISA Kit (ABIN4947610)

Murtaza, Hichami, Khan, Shimpukade, Ulven, Ozdener, Khan: "Novel GPR120 agonist TUG891 modulates fat taste perception and preference and activates tongue-brain-gut axis in mice." in: Journal of lipid research, Vol. 61, Issue 2, pp. 133-142, (2020) (PubMed).

Stygar, Sawczyn, Skrzep-Poloczek, Owczarek, Matysiak, Michalski, Mielańczyk, Bażanów, Ziora, Choręza, Doleżych, Karcz: "The Effects of Duodenojejunal Omega Switch in Combination with High-Fat Diet and Control Diet on Incretins, Body Weight, and Glucose Tolerance in Sprague-Dawley Rats." in: Obesity surgery, Vol. 28, Issue 3, pp. 748-759, (2018) (PubMed).

Guo, Han, Wang, Zhang, Liu, Zhang, Hu: "miR-200a regulates Rheb-mediated amelioration of insulin resistance after duodenal-jejunal bypass." in: International journal of obesity (2005), Vol. 40, Issue 8, pp. 1222-32, (2018) (PubMed).

Dolo, Yao, Li, Zhu, Shi, Widjaja: "Preserving Duodenal-Jejunal (Foregut) Transit Does Not Impair Glucose Tolerance and Diabetes Remission Following Gastric Bypass in Type 2 Diabetes Sprague-Dawley Rat Model." in: Obesity surgery, Vol. 28, Issue 5, pp. 1313-1320, (2018) (PubMed).

Wu, Yan, Cheng, Zhong, Liu, Zhang, Hu: "Deactivation of the NLRP3 inflammasome in infiltrating macrophages by duodenal-jejunal bypass surgery mediates improvement of beta cell function in type 2 diabetes." in: Metabolism: clinical and experimental, Vol. 81, pp. 1-12, (2018) (PubMed).

Chen, Xia, Liu, He, Zhang: "Selective Vagotomy Worsens Glucose Control After Ileal Transposition." in: Obesity surgery, Vol. 28, Issue 8, pp. 2494-2499, (2018) (PubMed).

Yan, Chen, Zhu, Lin, Pan, Li, Wang, Yang, Liu, Gong: "Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21." in: Frontiers in physiology, Vol. 9, pp. 191, (2018) (PubMed).

Wu, Cheng, Huang, Zhong, Liu, Hu: "Downregulation of lncRNA MALAT1 contributes to renal functional improvement after duodenal-jejunal bypass in a diabetic rat model." in: Journal of physiology and biochemistry, Vol. 74, Issue 3, pp. 431-439, (2018) (PubMed).

Zhong, Liu, Zhang, Zhang, Liu, Hu: "Alterations in gut microbiota during remission and recurrence of diabetes after duodenal-jejunal bypass in rats." in: World journal of gastroenterology, Vol. 22, Issue 29, pp. 6706-15, (2016) (PubMed).

Wang, Zhou, Quach, Lu, Gao, Xu, Zhu: "Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model." in: Obesity surgery, Vol. 26, Issue 4, pp. 797-804, (2016) (PubMed).

Qi, Ke, Liu, Liao, Ke, Liu, Wang, Lin, Zhou, Wu, Chen, Liu et al.: "Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3β pathway in an amyloid β protein ..." in: European journal of pharmacology, Vol. 783, pp. 23-32, (2016) (PubMed).

Balakumar, Prabhu, Sathishkumar, Prabu, Rokana, Kumar, Raghavan, Soundarajan, Grover, Batish, Mohan, Balasubramanyam: "Improvement in glucose tolerance and insulin sensitivity by probiotic strains of Indian gut origin in high-fat diet-fed C57BL/6J mice." in: European journal of nutrition, (2016) (PubMed).

Bak, Wewer Albrechtsen, Pedersen, Knop, Vilsbøll, Jørgensen, Hartmann, Deacon, Dragsted, Holst: "Specificity and sensitivity of commercially available assays for glucagon-like peptide-1 (GLP-1): implications for GLP-1 measurements in clinical studies." in: Diabetes, obesity & metabolism, Vol. 16, Issue 11, pp. 1155-64, (2015) (PubMed).

Cao, Cao, Liu: "Expression of cholecystokinin2-receptor in rat and human L cells and the stimulation of glucagon-like peptide-1 secretion by gastrin treatment." in: Acta histochemica, Vol. 117, Issue 2, pp. 205-10, (2015) (PubMed).

Hauge-Evans, Bowe, Franklin, Hassan, Jones: "Inhibitory effect of somatostatin on insulin secretion is not mediated via the CNS." in: The Journal of endocrinology, Vol. 225, Issue 1, pp. 19-26, (2015) (PubMed).

Target Details for GLP-1

Target: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Alternative Name: Glucagon Like Peptide 1