Interferon gamma ELISA Kit

Catalog No. ABIN4986919

Mouse Interferon gamma ELISA Kit Colorimetric assay for quantification of Mouse Interferon gamma.

Quick Overview for Interferon gamma ELISA Kit (ABIN4986919)

Target: Interferon gamma (IFNG)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.02-1.0 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details Interferon gamma ELISA Kit

Minimum Detection Limit: 0.02 ng/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Mouse IFN-γ Ligand

Sensitivity: 7 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma

Background: Interferon gamma (IFN-γ) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA (1). Produced by Tlymphocytes and natural killer (NK) cells, IFN-γ ?is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. Human IFN-γ?is reported to be active only on human and non-human primate cells (5). The biochemistry and biological activities of the interferons have been extensively reviewed (2-9). Mouse IFN-γ encodes a 155 amino acid (aa) residue precursor protein with a hydrophobic signal peptide that is cleaved to generate the 133 aa residue mature protein (10). In solution, IFN-γ has been shown to exist as a non-covalently associated homodimer with topological similarity to IL-10. Mouse IFN-γ shows approximately 40 % aa sequence identity to human IFN-γ and there is no cross-reactivity across species (11,12). A receptor for IFN-γ ?has been identified and its gene localized to chromosome 6 (13,14) Apparently the product of a single gene, the receptor is a single chain 90 kDa glycoprotein that shows a high degree of species-specific binding of IFN-γ?(15-18). Functionally, IFN-γ ?produces a variety of effects. Produced by CD8+, NK, gd,and TH1 T helper cells, IFN-γ ?has documented antiviral, antiprotozoal and immunomodulatory effects on cell proliferation and apoptosis, as well as the stimulation and repression of a variety of genes (19-22) he antiprotozoal activity of IFN-γ ?against Toxoplasma and Chlamydia is believed to result from indoleamine 2,3-dioxygenase activity, an enzyme induced by IFN-γ ?(23).The immunomodulatory effects of IFN-γ? are extensive and diverse. In monocyte/macrophages, the activities of IFN-γ?include: increasing the expression of class I and II MHC antigens, increasing the production of IL-1, platelet-activating factor, H2O2, and pterin, protection of monocytes against LAK cell-mediated lysis, downregulation of IL-8 mRNA expression that is upregulated by IL-2, and, with lipopolysaccharide, induction of NO production.Finally, IFN-γ?has been shown to upregulate ICAM-1, but not E-Selectin or VCAM-1, expression on endothelial cells.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy