IL-4 ELISA Kit

Catalog No. ABIN4986957

This Rat IL-4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat IL-4.

Quick Overview for IL-4 ELISA Kit (ABIN4986957)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.02-1.0 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details IL-4 ELISA Kit

Minimum Detection Limit: 0.02 ng/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Rat IL-4 Ligand

Sensitivity: 7 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4

Background: Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils (1-3). The cDNA sequence of rat IL-4 predicts a 147 amino acid (aa) residue precursor protein containing a 24 aa residue signal peptide that is cleaved to generate the 123 aa residue mature protein containing four potential N-linked glycosylation sites and seven cysteine residues (4-6). Mature rat IL-4 shares 60 % and 44 % amino acid sequence similarity with mouse and human IL-4, respectively (5,6). IL-4 has multiple immune response-modulating activities on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro (1-3). The biological effects of IL-4 are mediated by specific cell surface receptor complexes. Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13 (7, 8). In addition to the membrane-bound form of IL-4 R, a naturally occurring soluble form of IL-4 R has been identified in human and mouse biological fluids and in mouse cell culture supernates (9-11). Soluble IL-4 R has been to shown to bind IL-4 with high affinity in solution.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation