IL-4 ELISA Kit

Catalog No. ABIN4986958

Pig IL-4 ELISA Kit Colorimetric assay for quantification of Pig IL-4.

Quick Overview for IL-4 ELISA Kit (ABIN4986958)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Pig

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.08-5.0 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details IL-4 ELISA Kit

Minimum Detection Limit: 0.08 ng/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Swine IL-4 Ligand

Sensitivity: 39 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4

Background: Interleukin4 (IL-4), also known as B cellstimulatory factor1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3).It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four αhelix structure (4). Porcine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature porcine IL-4 shares 78 % , 59 %, 41 %, and 41 % aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human IL-4 is active on porcine vascular endothelial cells (5). IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 Rα and the common γ chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4Rα and IL13Rα1. The type II receptor also transduces IL-13 mediated signals. IL4 is primarily expressed by Th2biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by na?ve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8, 11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation